<b>A sensitive enzyme immunoassay specific for salmon </b><b>calcitonin </b>
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چکیده
منابع مشابه
A sensitive manual enzyme immunoassay for thyroxine.
We describe an immunoassay for thyroxine in serum. In the assay specific antibody covalently bonded to latex particles is used, along with horseradish peroxidase as the label, and o-phenylenediamine as the chromogen. The flexible protocol is designed for manual execution. Performance is similar to that of the highest-sensitivity thyroxine radioimmunoassays. Results correlate well with radioimmu...
متن کاملSensitive enzyme immunoassay for hepatitis B virus core-related antigens and their correlation to virus load.
A sensitive enzyme immunoassay (EIA) specific for hepatitis B virus core antigen (HBcAg) and hepatitis B e antigen (HBeAg) was developed. We designated the precore/core gene products as hepatitis B virus (HBV) core-related antigens (HBcrAg). In order to detect HBcrAg even in anti-HBc/e antibody-positive specimens, the specimens were pretreated in detergents. The antibodies are inactivated by th...
متن کاملRapid and sensitive chemiluminescent enzyme immunoassay for measuring tumor markers.
We developed a chemiluminescent enzyme immunoassay (CLEIA) to quantify such tumor markers as carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), CA19-9, and CA125. We used a novel chemiluminescent substrate, a derivative of 1,2-dioxetane phosphate (AMPPD), to measure alkaline phosphatase as a labeling enzyme to Fab' fragments of antibody. Regardless of the solid phase, i.e., polystyrene be...
متن کاملA rapid, sensitive enzyme-linked immunoassay for human thyrotropin.
In this enzyme-linked immunoassay for human thyrotropin (TSH) in unextracted serum we use 96-well immunoenzymometric assay plates, first coated with polyclonal antibody to TSH, then incubated with the serum samples and reacted with mouse monoclonal antibody to human TSH. After incubation with alkaline phosphatase-labeled antibody against mouse IgG, disodium p-nitrophenyl phosphate is added and ...
متن کاملA sensitive enzyme immunoassay for angiotensin II in serum.
A sensitive and specific enzyme immunoassay for measuring angiotensin II (AII) has been developed as a convenient alternative to a radioimmunoassay. An antiserum to AII was prepared using AII conjugated by carbodi-imide to rabbit serum albumin, and coated on to microwell plates. The labelled antigen was prepared from AII and horseradish peroxidase using the periodate method. This enzyme immunoa...
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1996
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.17.257